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Fig. 4. NOSTRIN, by means of its SH3 domain, interacts with multiple proteins. (A) GST-NOSTRIN was used in a pull-down assay using lysates of CHO-eNOS cells. GST alone served as control. (B) Co-immunoprecipitation from CHO-eNOS cells infected with SFV-NOSTRIN-GFP or SFV-GFP (control), using anti-GFP. (C) Lysates of COS cells transfected with dynamin cDNA for 24 hours (upper panel) or untransfected (lower) were subjected to precipitation with GST-NOSTRIN, GST-NOSTRIN-SH3, GST-NOSTRIN-{Delta}SH3 or GST alone. (D) Immobilized GST-NOSTRIN and GST were incubated with lysates of COS cells expressing wild-type dynamin (wt) or the dominant-negative mutant dynamin-K44A (K44A). Empty vector (-) served as control.





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