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Fig. 6. NOSTRIN, eNOS and N-WASP co-localize at actin filaments. CHO-eNOS cells were transiently transfected with cDNA encoding NOSTRIN (A-D), N-WASP-GFP (E-H), or NOSTRIN and N-WASP-GFP (I-P). Following fixation, cells were stained with anti-NOSTRIN (B,J,N), anti-eNOS (C,G,K) and phalloidin (O). (E,I,M) Fluorescence of GFP-fused N-WASP. (D,H,L,P) Merged images of each row. (A-D) Upon moderate expression, NOSTRIN localizes to vesicular and filamentous structures, where it co-localizes with eNOS. (E-H) When N-WASP-GFP is expressed alone, it is mainly diffusely dispersed in the cytoplasm and does not co-localize with eNOS. (I-L) Co-expression with NOSTRIN brings N-WASP-GFP to eNOS-positive, mostly filamentous structures. (M-P) NOSTRIN and N-WASP-GFP co-localize at structures running along phalloidin-labeled actin filaments. Bars, 10 µm.





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