QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. JIL-1 and lamin Dm₀ immunoprecipitation assays. (A) Immunoprecipitation (ip) of S2 cell lysate was performed using the lamin mAb HL1203 (lane 1) and the leech tractin control mAb 1H4 (lane 2) coupled to immunobeads or with immunobeads only (lane 3). The immunoprecipitations were analyzed by SDS-PAGE and western blotting using JIL-1 antiserum for detection. JIL-1 antiserum staining of S2 cell lysate is shown in lane 4. JIL-1 is detected in the lamin immunoprecipitation sample as a 160 kDa band (lane 1) but not in the control samples (lanes 2 and 3). (B) Immunoprecipitation (ip) of S2 cell lysate was performed using JIL-1 antiserum (lane 1) as well as a pre-immune serum control (lane 2) coupled to immunobeads or with immunobeads only (lane 3). The immunoprecipitations were analyzed by SDS-PAGE and western blotting using lamin mAb HL1203 for detection. Lamin mAb HL1203 staining of S2 cell lysate is shown in lane 4. Lamin is detected in the JIL-1 antiserum immunoprecipitation sample as a 76 kDa band (lane 1) but not in the pre-immune or beads-only control samples (lanes 2 and 3). The relative migration of molecular weight markers is indicated to the right in kDa.

Return to article