QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Effect of Nlp and ninein overexpression on Golgi organisation. (A) Tet-On U2OS stable cell lines carrying Myc-Nlp, Myc-ninein or EGFP-C-Nap1 were either treated with tetracycline (+Tet) or left untreated (–Tet). Cells were then grown for 5 hours, fixed and analysed by immunofluorescence microscopy. Overexpressed proteins were detected with anti-Myc antibodies or using EGFP fluorescence (green). The Golgi was detected using antibodies against the Golgi matrix protein GM130 (red). DNA was stained with DAPI (blue). (B) Expression of Myc-Nlp, Myc-ninein or EGFP-C-Nap1 was either induced in Tet-On U2OS stable cell lines with tetracycline (black bars), or the cells were left untreated (grey bars). Histograms show the percentage of cells with a fragmented staining for the Golgi marker GM130, calculated from three independent experiments in which 400-600 cells were counted. Error bars indicate standard deviations. (C) Myc-tagged Nlp and ninein were expressed in U2OS cells. These were then stained with Myc antibodies to detect the overexpressed protein (green) and counterstained with antibodies against -tubulin (red). Bars, 10 µm.

Return to article