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Fig. 2. Nlp and ninein associate with the dynein-dynactin complex. (A,B) U2OS cells expressing Myc-Nlp, Myc-ninein or EGFP-C-Nap1 were analysed by immunofluorescence microscopy. Overexpressed proteins were detected with anti-Myc antibodies or using EGFP fluorescence (green). Induced (+Tet) and control (–Tet) cells were counterstained with antibodies to (A) anti-p150Glued or (B) the dynein intermediate chain (DIC) (red). DNA was stained with DAPI (blue). Arrowheads indicate centrosomes. Bars, 10 µm. (C) Immunoprecipitation experiments were performed from extracts of HEK293T cells transfected with EGFP-tagged Nlp, ninein or C-Nap1 using anti-GFP antibodies. Immunoprecipitates were analysed by blotting with antibodies against GFP, the dynactin subunits p150Glued and p50-dynamitin, and pericentrin.





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