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Fig. 4. The dynactin-binding domains of Nlp and ninein are required for the Golgi fragmentation phenotype. (A,B) U2OS cells were transfected with EGFP-tagged Nlp, Nlp{Delta}EF-h I ({Delta}1-38aa), EGFP-Nlp{Delta}EF-h I-II ({Delta}1-350aa), and the Nlp N- and C-terminal domains, or the EGFP-tagged N-terminal domain of ninein. Expressed proteins were detected using EGFP fluorescence (green). (A) The Golgi was stained with antibodies against GM130 (red), transfected cells are marked by an asterisk. (B) the dynactin complex was stained with antibodies against p150Glued (red). DNA was stained with DAPI (blue). Bars, 10 µm. (C) A schematic representation of the Nlp and ninein deletion mutants, and a summary of their effects on p150Glued and the Golgi.





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