spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Plk1 regulates the association of Nlp with the dynein-dynactin complex. (A) U2OS cells were transfected with EGFP-Nlp or EGFP-Nlp{Delta}8 (green) together with either Plk1K82R or Plk1T210D, and the localisation of p150Glued (red) was analysed by immunofluorescence microscopy. Bar, 10 µm. (B) Nlp was immune precipitated from extracts of `Tet-On' Myc-Nlp-expressing cells using antibodies to the Myc-epitope; control precipitations were performed using a non-specific IgG fraction. Samples of the cell lysate, unbound, and bound fractions were western blotted for Nlp and p150glued. (C) Total cell extracts were incubated with the GST-Nlp N terminus, GST-Nlp N terminus mock-treated with Plk1 and GST-Nlp N terminus phosphorylated by Plk1. Bound fractions were analysed by immunoblotting using antibodies to p150Glued and Plk1 (left panel), and Coomassie Brilliant Blue staining (right panel).





Right arrow Return to article