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Fig. 6. NIH-3T3 fibroblasts do not migrate towards hyaluronan. (A) NIH-3T3 fibroblasts transfected with vector alone or human CD44s were assayed for their ability to migrate towards DCS (0.25-10.00% gradient) or umbilical cord hyaluronan (0-400 µg/ml gradient) as described in Fig. 1. (B) Cells were subjected to flow cytometry to monitor expression of murine CD44 using monoclonal antibody KM201 (blue), expression of human CD44 using monoclonal antibody E1/2 (red) and FITC-HA binding (green) as described in Fig. 2.





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