spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Characterization of YFP-IL-6 and binding to gp130. (A) YFP-IL-6 consists of the signal sequence for secretion (black, 29 amino acids) followed by the YFP moiety (yellow, 238 amino acids) and mature human IL-6 (grey, 184 amino acids). (B) Ba/F3-gp130-IL-6R{alpha} cells were left unstimulated or were stimulated for 15 minutes with 20 ng/ml IL-6 or 40 ng/ml purified YFP-IL-6. Phosphorylation of STAT3 was analysed by immunoblotting of cellular lysates using an antibody against tyrosine-phosphorylated STAT3. Equal loading was controlled using a STAT3 antibody. (C) COS-7 cells were transfected with an expression vector encoding gp130-CFP. 48 hours after transfection, confocal images from living cells incubated in a perfusion chamber at 37°C were taken using the YFP and CFP channels of the confocal microscope. Cells were stimulated with YFP-IL-6 (40 ng/ml) and sIL-6R{alpha} (1 µg/ml) (upper row) or with YFP-IL-6 alone (lower row). Confocal images were taken at the time points indicated. Bar, 10 µm.





Right arrow Return to article