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Fig. 1. Branches of the Nek family showing (A) the Nek2 clade, (B) the Nek6/Nek7 clade and (C) the higher-plant clade. The sequence alignment and bootstrapped consensus trees were generated using ClustalW (http://www.ddbj.nig.ac.jp/search/clustalw-e.html). Outputs were generated in Treeview (v1.6.6). The proteins included in each branch were derived from a larger tree, generated using 81 Nek sequences and four non-Nek kinases (M.R.M. and L.M.Q., unpublished). The baseline bootstrap values represent the value for the given group as a clade on the larger tree. Abbreviations: An, Aspergillus nidulans; At, Arabidopsis thaliana; Ce, Caenorhabditis elegans; Ci, Ciona intestinalis; Cr, Chlamydomonas reinhardtii; Dd, Dictyostelium discoideum; Dm, Drosophila melanogaster; Dr, Danio rerio; Hs, Homo sapiens; Mm, Mus musculus; Os, Oryza sativa; Pf, Plasmodium falciparum; Sc, Saccharomyces cerevisiae; Sp, Schizosaccharomyces pombe; Tb, Trypanosoma brucei; Tp, Tetrahymena pyriformis. Colours: blue, vertebrates; orange, fungi; green, plants and green algae; red, Ciona; black, C. elegans; grey, Dictyostelium; brown, Drosophila; purple, Plasmodium.

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