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Fig. 4. Slow muscle development is disrupted in ryanodine treated embryos. Embryos were treated with ryanodine just prior to 17 hpf and fixed and stained at 24 hpf. Immunostaining with antibody F59 was performed to reveal slow muscle fibres in (A,D) control (B,E) 10 µM (C,F) 50 µM ryanodine treated embryos. Bars (A-C) 50 µm and (D,E) 10 µm. Insets (B,C) show cross section of slow muscle myosin in anterior trunk of embryos. (E,F) In ryanodine treated embryos striations (arrows with asterisks) were evident and myofibrils (arrows) that had not aligned into bundles were observed.





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