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Fig. 5. Acetylcholine generates calcium transients and regulates slow muscle development. Embryos were loaded with Oregon Green BAPTA dextran and incubated in (A) embryo medium or (B) AChR blocker {alpha}-bungarotoxin (0.5 µM) for 30 minutes prior to imaging. All calcium signals were inhibited in the presence of the acetylcholine receptor blocker {alpha}-bungarotoxin between 18-20 hpf (n=18). Tailcut embryos were incubated in (C) embryo medium, or (D) AChR blocker {alpha}-bungarotoxin (0.5 µM) from 16 hpf and fixed at 24 hpf. Immunostaining with antibody F59 revealed slow muscle fibres. Inset shows cross section to reveal slow muscle distribution in one half of embryo. Bars 20 µm.





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