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Fig. 2. Shrm controls apical morphology in MDCK cells. (A-C) A mixture of T23 and ShrmL cells were grown on Transwell filters in the absence of doxycyclin and stained to detect Shrm (green), ZO-1 (red) and E-cadherin (B,C). Asterisks indicate constricted, ShrmL-expressing cells and dots indicate stretched, non-expressing cells. Panels B and C show single optical sections of E-cadherin staining at the basal and apical surfaces, respectively. (D) Scatter plot of apical (ap) and basal (bsl) areas of ShrmL expressing (1), neighboring wild-type (2) and non-neighboring, wild-type (3) cells in mixed populations of ShrmL expressing and wild-type T23 cells. Each point represents one cell. (E-G) Clusters of increasing numbers of ShrmL-expressing cells (stained to detect Shrm) show the configuration of cells at the level of the AJC. (H-J) Cysts generated from control cells (H) or induced ShrmL cells (I and J) were stained to detect Shrm (green), ZO-1 (red) and F-actin (blue). Arrows denoted cells with constricted apical surfaces. Scale bar represents 15 µm in all panels.

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