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Fig. 5. The activity of myosin II and Rock are required for Shrm-induced cell shape changes. (A,B) Control cells were treated with blebbistatin (bleb) or Y27632 and stained to detect ZO-1. (C-H) Induced ShrmL cells (C,E,G) or Endo-ShrmC cells (D,F,H) were treated with DMSO (C and D), 100 µM blebbistatin (E and F), or 10 µM Y-27632 (G,H) and stained to detect Shrm (green) and ZO-1 (red). Arrowheads denote positions of the X-Z projections shown beneath each panel. (I-K) Control cells (I) or ShrmL-expressing cells (J and K) were transfected with expression vectors for either GFP-RhoA (I and J) or GFP-RhoA19N (K) and stained to detect GFP, Shrm and E-cadherin. Merged images (I-K) show GFP (green) and ShrmL (red). Individual fluorescent signals for GFP (I'-K') and E-cadherin (I''-K'') are included. Arrows indicate transfected cells. In all panels, scale bar represents 15 µm.
