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Fig. 1. Analysis of cell movement. (A) Region of under agarose assay that is analyzed during the cell tracking experiments. The dotted box represents the region being recorded, which is located adjacent to the neutrophil-containing well. (B) Example of how a cell is tracked in the cell tracking assay. The neutrophil-containing well is visible on the left, and the cells are migrating towards a source of fMLP located off-screen to the right. The path taken by the cell is indicated by the white, curved line. The distance each cell moves between each time point (dotted line) is calculated, and then the time points are summed to give the total distance moved by the cell ( ${Sigma}$ _dist). Next, the total distance the cell migrates towards the chemoattractant was calculated. This value is equal to the displacement of the cell along the x-axis ( ${Delta}$ _x). Speed was calculated by dividing ${Sigma}$ _dist by the duration of the experiment. Chemotactic index (C.I.) was calculated by dividing ${Delta}$ _x by ${Sigma}$ _dist.

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