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Fig. 10. LFA-1 activation on cells moving on serum towards single gradients of fMLP or IL-8. (A) Relative activation of LFA-1. Values are expressed as fold-activation, compared with fMLP. (B) Total LFA-1 on cells, as measured using an activation-independent antibody. (C) DIC and fluorescent microscopy showing the localization of active LFA-1 on cells moving to fMLP (top) or IL-8 (bottom). Integrin data represent only the integrins in contact with the substratum, and is expressed as fluorescent intensity relative to fMLP. Results are shown as mean±s.e.m., n=minimum of 5. *P<0.05 compared with fMLP.





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