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Fig. 11. Integrin activation on cells migrating on serum in competing gradients of fMLP and IL-8. Cells are either uninhibited (UT) or treated with the p38 MAPK inhibitor SB203580. (A) Relative Mac-1 activation. Values are expressed as fold-activation, compared with UT. (B) Relative LFA-1 activation. Values are expressed as fold-activation, compared with UT. (C) Total Mac-1 and LFA-1 on cells, as measured using activation-independent antibodies. (D) Effect of blocking antibodies against Mac-1 and LFA-1 on chemotaxis in the hierarchy. (E) Effect of p38 MAPK inhibition on chemotaxis in the hierarchy, and the roles of LFA-1 and Mac-1 in mediating this chemotaxis. Integrin data represents only the integrins in contact with the substratum, and are expressed as fluorescent intensity relative to fMLP, UT. Results are shown as mean±s.e.m., n=minimum of 5. *P<0.05 compared to untreated.
