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Fig. 4. Chemotaxis of neutrophils to fMLP, IL-8 and in the hierarchy on substrata of serum protein (serum), fibrinogen (Fbgn), or fibrinogen + ICAM-1 (Fbgn + ICAM-1). (A) Effect of different substrata on the number of cells migrating towards (white bars) and away (black bars) from fMLP and IL-8. (B) Chemotactic index (C.I.) of cells migrating to fMLP and IL-8. (C) Speed of migration to fMLP and IL-8. (D) Tracks plotting the migration of ten representative cells to fMLP (left) or IL-8 (right) on fibronectin + ICAM-1. Scale is in µm. (E) Patterns of cell migration in competing gradients of fMLP and IL-8. The number of cells migrating to fMLP and IL-8 (x-axis), as well as the number of cells migrating perpendicular to the chemoattractants (y-axis) are shown. Results are shown as mean±s.e.m., n=6 for panels A,D,E; n=4 for panels B,C. *P<0.05 compared with migration on serum protein.





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