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Fig. 9. Mac-1 activation on cells moving on serum towards single gradients of fMLP or IL-8. Cells are either uninhibited (UT) or treated with the p38 MAPK inhibitor SB203580. (A) Relative activation of Mac-1. Values are expressed as fold-activation, compared with fMLP, UT. (B) Total Mac-1 on cells, as measured using an activation-independent antibody. (C) DIC and fluorescent microscopy showing the localization of active Mac-1 on cells moving to fMLP, with and without p38 MAPK inhibition. (D) Effect of p38 MAPK inhibition on chemotaxis to fMLP and IL-8. Integrin data represents only the integrins in contact with the substratum, and are expressed as fluorescent intensity relative to fMLP, UT. Results are shown as mean±s.e.m., n=minimum of 5. *P<0.05 compared with untreated.
