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Fig. 5. Influence of IFN{gamma} on permeability to bacterial lipopolysaccharide. (A) Control or IFN{gamma}-treated T84 monolayers (10 ng/ml; 48 hours), were washed with HBSS and 0.2 mg/ml FITC-LPS added to the apical surface. The flux of FITC-LPS into the basolateral chamber was measured after 4 hours by fluorimetry and shown in µg/hour/cm2 as mean±s.e.m. of five to seven monolayers in each group. (B) Effect of inhibitors of endocytic pathways on FITC-LPS flux. Monolayers were exposed to 10 µg/ml chlorpromazine (CPZ), 10 µM LY-294002 or 3 µg/ml filipin for 15 minutes prior to addition of FITC-LPS. The IFN{gamma}-mediated increase in FITC-LPS flux in the presence of inhibitors was not significantly different to that observed in the absence of inhibitors. Data are mean±s.e.m. of three to six monolayers in each group.





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