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Fig. 2. Localization of LAMP1-GFP fusion protein expressed in transfected murine fibroblasts. Immortalized skin fibroblasts derived from wild-type (A-I) or pale ear (J-O) mice were transfected with a plasmid encoding human LAMP1 fused to GFP (A,D,G,J,M and green in C,F,I,L,O). One day after transfection, the cells were fixed/permeabilized and stained with a species-specific antibody to murine Lamp1 (B,K and red in C,L) or an antibody to EEA1 (H and red in I). Alternatively, cells were allowed to internalize Texas-Red-conjugated dextran, followed by a 4-hour chase period to ensure specific labeling of mature lysosomes (E,N and red in F,O). Bar, 20 µm. The top-left region of panels A-C, which displays staining of endogenous Lamp1 but not of LAMP1-GFP, corresponds to part of an untransfected cell. Magnified views of selected areas are shown in the insets.

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