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Fig. 7. Effect of BMP antagonists on cumulus cell apoptosis. OOX were cultured with 10% v/v BMP-15 in the presence of increasing doses of follistatin (0-100 µg/ml) (A), and OOX were cultured with 10 ng/ml BMP-6 in the absence or presence of a high neutralizing dose of 20 µg/ml of a BMP-6 neutralizing antibody (NAb) (B). Suppression of cumulus cell apoptosis by BMP-15 was antagonized by follistatin. The NAb effectively antagonized the anti-apoptotic effects of BMP-6. Points and bars represent average percentage of apoptotic cumulus cells (mean ±s.e.m.). Values from points with different labels; a,b,cdiffer significantly (P<0.001).





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