JCS02646 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. TIRF microscopy of live COS-7 cells transfected with GFP-Dvl2, revealing undirected movements of GFP-Dvl2 puncta within the evanescent field near the plasma membrane.

• Movie 2 -

  Movie 2. TIRF microscopy of live COS-7 cells transfected with GFP-Dvl2, depicting the fusion of two GFP-Dvl2 puncta (see also frames shown in Fig. 6).

• Movie 3 -

  Movie 3. TIRF microscopy of live COS-7 transfected with GFP-Dvl2 and clathrin-DsRed, demonstrating that the Dvl2 and clathrin puncta are distinct (see also frame shown in Fig. 7).