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Fig. 9. Dynamic exchange of DIX-domain proteins between punctate and cytosolic pools. Average-sized puncta of (A-C) GFP-Dvl2, (E-G) GFP-DIX or (I-K) GFP-Axin were highlighted with white boxes and bleached, and fluorescence recovery was monitored for the following 3 minutes (at 5-second intervals); unbleached neighbouring puncta (yellow boxes) served as internal controls. (D,H,L) Graphs showing mean fluorescence intensities within the boxes indicated (of bleached puncta, or unbleached controls), were normalised to the mean fluorescence intensity of the whole cell.





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