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Fig. 1. Localisation of ectopically expressed Dvl-2. (A-D) Localisation of transiently expressed HA-Dvl-2-EGFP (green) in HEK293 cells (A-B, nuclei counterstained with ToPro III), MDCK cells (C, nuclei counterstained with ToPro III) and CHO cells (D, no nuclear counterstain). (E-F) Localisation of transiently expressed HA-Dvl-2-ER (green) in MDCK cells. Cells were fixed 1 hour after re-feeding with medium containing ethanol vehicle (E) or 1 µM ß-estradiol (F). Bar, 15 µm.