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Fig. 2. Agonist-induced endocytosis of LPA1 is inhibited in ß-arrestin 1/2 double knockout mouse embryo fibroblasts. (A) Wild-type MEFs were transiently transfected with plasmids encoding either FLAG-tagged LPA1 or HA-tagged ß2AR and then incubated in the presence or absence of agonist (10 µM LPA or 20 µM isoproterenol, respectively) for 30 minutes prior to indirect immunofluorescence localization of the receptor proteins either in the presence or absence of detergent permeabilization. (B) ß-arrestin 1/2 double knockout MEFs were transiently transfected with plasmids encoding either FLAG-tagged LPA1 or HA-tagged ß2ARs and incubated in the presence or absence of agonist, as above, prior to indirect immunofluorescence localization of the receptor proteins. Bar, 10 µm.





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