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Fig. 3. The C-terminal region of Mesd is required for interaction with LRP6. (A) Schematic representation of Mesd mutants. (B) Binding analyses of 125I-Mesd and its mutants (5 nM) to LRP6-transduced HT1080 cells. Assays were carried out for 3 hours at 4°C in the absence or presence of 500 nM Mesd or its mutants as indicated. Values are the means of triple determinations with the s.d. indicated by error bars.





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