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Fig. 6. LRP6 is not a constitutively active endocytosis receptor. (A) Anti-HA IgG binding to cell surface HA-tagged LRP6. Binding of 125I-anti-HA IgG (1 nM) to LRP6-transduced HT1080 cells and the control cells was carried out for 90 minutes at 4°C. (B) LRP6 endocytosis. LRP6-transduced HT1080 cells, mLRP4-transfected CHO cells and mLRP4tailess-transfected CHO cells were incubated with 1 nM 125I-anti-HA IgG at 4°C for 90 minutes, and then incubated at 37°C for the indicated times. The amount of internalized anti-HA IgG was determined. Values are the means of triple determinations with the s.d. indicated by error bars.





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