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Fig. 4. FA topographs (A) and 3D-reconstruction (B) showing the wedge-shaped morphology and the finger-like array of microfilaments at the distal end of the SF. (C) Side view of the same FA with Y and Z dimensions at the same scale. The height of the FA increases towards the SF-associated end at an angle of approximately 3°. From the AFM topograph the area (1.9x106 nm2) and the volume (1.2x108 nm3) of the FA was determined. (D) Location of X-Z cross sections taken through the FA topograph every 500 nm in the Y direction. (E) Overlay of the cross section demonstrates that the FA structure is low and wide at the front and high and narrow at the SF-associated end. (F) Graphs showing the relative changes in FA height, width and cross-section area moving from the distal to the proximal end of the FA. (G) Schematic representation of the 3D-array of microfilaments in FAs. Height scale in A, 200 nm.





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