QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. F-actin and paxillin fluorescence images of an FA in a de-roofed cell (A-C). Corresponding topograph (D) and fluorescence/height overlay image (E). The circular area with decreased F-actin staining (A, asterisk) corresponds to an area of decreased height in the AFM topograph (D). This defect may be the result of mechanical disruption of the FA structure during de-roofing. In contrast to the F-actin signal, the paxillin signal in this area is not decreased (B). (F) A line scan through the overlay image (E) demonstrates a correlation between the height and F-actin signal but not the paxillin signal. The shaded area (light green) indicates a higher relative fluorescence intensity of paxillin-YFP compared to the F-actin staining. As the disruption of FA architecture down to a height of less than 50 nm does not decrease the paxillin signal, the paxillin localization must be restricted to a membrane-proximal region of the FA. F-actin-containing structures, on the other hand, must be localized predominantly in the membrane-distal half, as disruption of the FA structure down to about 50 nm reduces F-actin staining to almost background levels. Bar, 500 nm.

Return to article