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Fig. 1. Endocytosis is decreased in dab2^-/- mutants. (A,B) EM analysis of the VE of (A) control (+/) and (B) dab2^-/- (–/–) embryos at E6.5. Electron-dense vesicles are indicated by red arrows. (C) HRP uptake in control and dab2^-/- embryos at E7.5. Mutant embryos showed reduced uptake of HRP compared with control embryos. (D-I) TR-Tf uptake in E6.5 and E7.5 embryos. (D) In E7.5 embryos, Tf uptake is reduced in dab2^-/- embryos compared with control littermates. (E,G) Dark-field images of E6.5 wild-type (E) and mutant (G) embryos. (F,H) Fluorescent images of the same embryos show decreased uptake in the VE of the mutant embryo. (F',H') Higher magnification images of regions indicated in F, H show a reduction in endosomal labeling in the dab2^-/- VE. (I) TR-Tf uptake is significantly decreased in dab2 mutant embryos (P<0.001). Fluorescent images were taken at the same exposure. The average pixel intensity was determined within a central region of each embryo and plotted as a scatter plot (closed circles). The mean intensity (open circle) and standard error (bars) were calculated for each genotype, and statistical significance was determined using the Mann-Whitney test. Bar, 2 µm. PE, parietal endoderm; BB, brush border; VE, visceral endoderm.

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