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Fig. 3. ARH is absent from the VE. (A-C) Whole-mount immunofluorescence for Dab2 and ARH of E6.5 wild-type embryos. (A) Dab2 protein was detected by indirect fluorescence in the VE surrounding the entire embryo. (B) ARH was absent in the VE, but was detected in the PE. (C) As shown in the merged image, Dab2 and ARH are expressed in distinct tissues of the E6.5 embryo. The magnified region shows the low extent of colocalization (as indicated by yellow pixels) between Dab2 and ARH.





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