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Fig. 5. Abi1 and WAVE2 are required for CSF-1-induced F-actin rich membrane protrusions in macrophages. (A) Abi1, WAVE2 and ß-actin expression in Abi1 shRNA-treated cells (two different clonal populations shown) was analyzed by western blotting with the appropriate antibodies and compared to mock shRNA-treated cells. Quantification of Abi1/ß-actin and WAVE2/ß-actin signal intensity ratios is shown below. (B) Mock- and Abi1-shRNA-treated cells were fixed and stained for Abi1 as described in Materials and Methods. Bar, 10 µm. (C) Mock shRNA-treated cells and two independent clones with reduced Abi1/WAVE2 expression (Abi1 shRNA) were treated with 20 ng/ml CSF-1 for 5 minutes and the ability of cells to form F-actin-rich membrane protrusions in response to CSF-1 was scored as described in Fig. 3 and expressed as a percentage of the CSF-1 stimulation observed in mock shRNA-treated cells; n=3, *P<0.05 compared to mock shRNA-treated cells.
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