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Fig. 6. WAVE2 and Abi1 are required for CSF-1-induced cell migration in macrophages. (A) Non infected (NI) and mock shRNA-treated cells showed no significant difference in cell migration in response to 20 ng/ml CSF-1 using a transmigration chamber assay as described in Materials and Methods, n=4. (B,C) Using this assay, cell migration in response to CSF-1 was determined in WAVE2 shRNA-treated cells (B) or Abi1 shRNA-treated cells (C) and compared with that of mock shRNA-treated cells. CSF-1-stimulated migration of each cell population was compared with the corresponding unstimulated condition and expressed as a percentage of the mock shRNA-treated cells; n=3 for B and C, *P<0.05 compared to mock shRNA-treated cells.





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