JCS02652 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Pav-KLP RNA levels are not elevated in UAS-tum expressing stripes. In-situ hybridisation with Pav-KLP antisense RNA probes, followed by staining with antibodies to reveal UAS-tum expression stripes (in green, B) and lacZ (to identify homozygous mutant embryos, not shown) detects endogenous elevated RNA levels in the CNS of tum^DH15 mutant embryos (A) but not in the stripes of cells expressing UAS-tum (B). All embryos are oriented with their anterior to the left. Bar, 100 mm.

• Supplemental Figure 2 -

  Fig. S2. Expression of UAS-tum^DYRL causes a late-cytokinetic defect in some tum mutant cells. Tum^DYRL protein (shown in A, and red in panel B) is occasionally (4/56 post anaphase cells) found in rings embedded in the F-actin cortex in recently divided cells. Panel B includes DNA in blue and F-actin in green. Bar, 5 mm.

• Supplemental Figure 3 Part1
• Supplemental Figure 3 Part2 -

  Fig. S3.  Loss of Tum results in mis-localisation of other cytokinetic furrow components in mutant embryos. This figure repeats Fig. 3 but includes greyscale images for all colour channels.  The letters on the left refer to the merged images shown in Fig. 3, with DNA and other channels labelled beneath the image. 

• Supplemental Figure 4 -

  Fig. S4. Transgene deletions demonstrate that a functional GAP domain is required for Tum cytokinetic function. This figure repeats Fig. 5 but includes greyscale images for all colour channels.  The letters identify the same panels from Fig. 5 with the additional channels labelled underneath.

• Supplemental Figure 5 -

  Fig. S5. Transgene deletions demonstrate that interaction with Pbl and Pav-KLP are required for Tum cytokinetic function. This figure repeats Fig. 6 but includes greyscale images for all colour channels. Lettering from Fig. 6 is repeated in this figure with additional greyscale panels labelled underneath.