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Fig. 4. Elevated Pav-KLP protein levels occur in stripes of cells expressing all but the UAS-tum^Pav transgene. In these experiments transgene-derived Tum localisation was detected with a myc antibody (shown in green), and Pav-KLP is shown in red. (A-C) The arrowhead in C indicates two anaphase cells with Pav-KLP and Tum colocalising at the furrow sites in a prd-GAL4-induced expression stripe. Midbody remnants containing both proteins are also evident. In panel C DNA staining is blue and the bracket marks the approximate boundary of the prd expression stripe. Pav-KLP is detected in stripes of mutant cells expressing Tum (D-F), Tum^EIE (G-I), Tum^YRL (J-L), Tum^Pbl (P-R), but not Tum^Pav (M-O). The Pav antibody detects sequestered Pav-KLP protein in the germ line (arrowhead, N) of Tum^Pav-expressing embryos but detects no Pav-KLP in the stripes of Tum^Pav expression. Bar, 5 µm (A-C); 100 µm (D-R).

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