Supplemental Figure 1
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Fig. S1.
Dominant-negative Rac and Cdc42 do not alter the effect of IMD expression in
serum-starved Swiss 3T3 cells. Constructs encoding myc-IMD (A-C), myc-IMD + HA
N17-Rac (D-F) or myc-IMD + HA N17-Cdc42 (G-I) were microinjected into
serum-starved quiescent Swiss 3T3 cells. Cells were treated for indirect FITC
(green) localisation of MIM-B constructs with anti-myc antibody (A,D,G) and
F-actin with TRITC-coupled (red) phalloidin (B,E,H). Merged images (C,F,I) are
presented. Expression of HA-tagged constructs was confirmed with anti-HA
antibody (not shown). Bar, 20 µm.
