spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. MIM-B protein is widely expressed and is not downregulated in metastatic cell lines. (A) Characterisation of anti-MIM-B antibody. (Left) Lysates from mouse brain (L) were used to immunoprecipitate endogenous MIM-B using pre-immune serum (PI) or affinity-purified anti-MIM-B antibody (Pur) and analysed by immunoblotting with anti-MIM-B antibody. (Right) Lysates from COS-7 cells transfected with constructs expressing myc-MIM-B, myc-MIM-B {Delta}IMD ({Delta}IMD) or myc-IMD (see Fig. 1) were analysed by immunoblotting with anti-MIM-B antibody and reprobed with anti-myc antibody. Positions of molecular size markers are indicated in kDa. (B) Tissue distribution of MIM-B. 50 µg lysates from various mouse tissues were separated on SDS-PAGE and analysed by immunoblotting with anti-MIM-B antibody. SK, skeletal. (C) MIM-B expression is not downregulated in metastatic cell lines. Expression of endogenous MIM-B was studied by immunoblotting with anti-MIM-B antibody in non-metastatic (-) or metastatic (+) cell lines from bladder (top), prostate or breast (bottom). Tubulin was subsequently probed as a loading control. This result is representative of three independent experiments.





Right arrow Return to article