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Fig. 3. The IMD of MIM-B is required for MIM-B-induced actin-rich membrane protrusions in serum-starved Swiss 3T3 cells. Constructs (see Fig. 1) encoding myc-MIM-B (A-C), myc-MIM-B {Delta}234 (D-F), myc-MIM-B {Delta}WH2 (G-I) or myc-IMD (J-L) were microinjected into serum-starved quiescent Swiss 3T3 cells. Cells were treated for indirect FITC (green) localisation of MIM-B constructs with anti-myc antibody (A,D,G,J) and F-actin with TRITC-coupled (red) phalloidin (B,E,H,K). Merged images (C,F,I,L) are presented. Boxed image in C is an enlargement of the merged image. Bar, 20 µm.





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