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Fig. 2. Ablation of PACRG AB produces a slow-growth phenotype. Q-PCR data (A,C,E) and representative growth curves (B,D,F) of cells undergoing RNAi of TbPACRG A (A,B), TbPACRG B (C,D) or TbPACRG AB (E,F). A reduction of mRNA signal of TbPACRG A (black bars) was seen in RNAi TbPACRG A-treated cells (A). However, there was no alteration in growth kinetics in TbPACRG A RNAi-treated cells (B, triangles) compared to non-induced controls (B, squares). A reduction of mRNA signal of TbPACRG B (grey bars) was observed in RNAi TbPACRG B-induced cells (C), but again, no alteration in growth kinetics was observed compared to the control (D). A reduction of mRNA signal of TbPACRG A (black bars) and TbPACRG B (grey bars) was observed in TbPACRG AB RNAi-treated cells (E) with the representative growth curve (F) showing a growth defect starting 72 hours after induction (triangles) compared to non-induced controls (squares). For the growth curves (B,D,F), cells were maintained in log phase by diluting the culture every 48 hours. Q-PCR values are percentages (mean±s.e.m.) of the control cell signal (A,C,E).
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