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Fig. 7. GFP-TbPACRG fusion proteins localise to the axoneme. Phase-contrast microscopy (A,C,E,G) and fluorescence (B,D,F,H) images of detergent-extracted trypanosome cytoskeletons. DNA is labelled with DAPI (blue), and GFP is identified by anti-GFP polyclonal antibody (green). (A,B) Cells expressing GFP-TbPACRG A. (C,D) Cells expressing GFP-TbPACRG B. (E,F) Cells expressing GFP-TbPACRG A where the PFR is identified by the L8C4, anti-PFR, monoclonal antibody (red). Note the GFP signal extends proximal to the PFR staining (F, arrow). (G,H) Cells expressing GFP-TbPACRG A where the distal end of the basal body is identified by the BBA4 monoclonal antibody (red). Bar, 5 µm.





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