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Fig. 2. Subcellular localization of EGFP-TIAR hybrid proteins. (A) Schematic representation of TIAR protein and TIAR mutants fused to EGFP protein. The amino acids bordering the different domains composing TIAR are indicated. The dotted lines indicate the deleted region in the different mutants. (B) Subcellular distribution of GFP and GFP-TIAR hybrid proteins (represented in A) were analyzed by epifluorescence microscopy. Cell nuclei were stained with DAPI. (C) Ratios of cytoplasmic (C) and nuclear (N) fluorescence observed for GFP alone or fused with TIAR or relevant domains of TIAR. These ratios are the means±s.d. and were calculated as described in the Materials and Methods.





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