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Fig. 8. TIAR and TIA-1 subcellular localization relies on the same sequence determinants. (A) Schematic representation of the TIA-1 constructs. The amino acids bordering the different TIA-1 domains are indicated. The dotted lines indicate the deleted region in the different mutants. (B,C) Subcellular distribution of GFP-TIA-1, GFP-TIA-1 mutants and NPc-RRM3(TIA-1)-NLS-FLAG described in A. The experiment was performed as described in Fig. 2B and Fig. 4C and ratios of cytoplasmic and nuclear fluorescence were calculated as previously described.





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