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Fig. 3. Localization of antibodies to the chromatin proteins in the telomeric regions of polytene chromosomes 2R (A-C,I,K,L), X (DH,J,M,O) and 3L (N). Capping proteins: (A) SUUR, (B) SU(VAR)3-7 and (C) HP2 localize to the chromosome tip. (D) H4AcK12 is not detected in telomeres. HeT-A/TAHRE/TART repeat recruits typical euchromatic proteins: (E) JIL-1, (F) Z4, (G) H3Me3K4 and Z4, both of which co-localize within this region. No productive transcription is detected within the HeT-A/TAHRE/TART region, since no signal for phospho-serine5 PolIIo is seen (H). Upon simultaneous immunodetection of cap- and HeT-A/TAHRE/TART-array-specific proteins, no overlap of the two signals is observed: HP2 and Z4 (I), HP1 and JIL-1 (J), HP1 and H3Me3K4 (K), HP1 and H3Me3K9 (L,M). (N) H3Me3K9 is detected within the HeT-A/TAHRE/TART region of Su(var)20503/Su(var)20505 larvae. (O) PC protein, recruited to the TAS repeats, and Z4 do not merge perfectly within the region of TAS repeats. Dashed lines, TAS repeats; solid lines, marker regions.





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