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Fig. 6. Effect of CYP 2C9 overexpression on endothelial cell invasion and tube formation. Human endothelial cells infected with CYP 2C9 sense (2C9) or antisense (CTL) viruses were seeded on Matrigel-coated Transwell filters (pore size 8 µm). Twenty-four hours after seeding the migrated cells were counted. Effects of (A) sulfaphenazole (Sulfa, 30 µmol/l) and 14,15-epoxyeicosa-5(Z)-enoic acid (EEZE, 10 µmol/l) and (B) the non-selective matrix metalloprotease inhibitor GM 6001 (GM, 30 nmol/l) on CYP-2C9-induced endothelial cell invasion. (C) Representative photographs and bar graph showing the effect of EEZE on tube formation by human endothelial cells infected with CYP 2C9 sense (2C9) or antisense (CTL) adenoviruses and seeded on fibrin gels. Tube formation was assessed after 14 days and total tube length calculated using a computer-assisted microscope. The bar graphs summarize data (mean±s.e.m.) obtained in four to five independent experiments; ^*P<0.05, ^**P<0.01, ^***P<0.001 vs levels in the respective controls (CTL).

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