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Fig. 1. Diagram of reporter constructs. (A) Diagram of the full length EPMH reporter (above). Native EP1 procyclin domains (from N to C terminus) are: signal sequence, black box; mature N-terminal domain, white box, EP repeat domain, stripped box; GPI attachment peptide, gray box. The single EP1 N-glycosylation site (lollipop) and the C-terminus of the EPMH{Delta}gpi reporter (arrowhead) are indicated. The position (codon 50) and sequence of the inserted cassettes are indicated (below). The insert sequence is in upper case, the methionine and HA cassettes are highlighted in black, and flanking native sequences are in lower case. (B) Domains and N-glycosylation (lollipops) patterns of the p67HP and BiPNHP GPI1 reporters: native signal sequences, black boxes; ectoplasmic domains, white boxes. The C-terminal HA and EP procyclin GPI-peptide sequences are indicated by dots. BiPNHP and p67HP contain codons 1-415 and 1-616 of the native sequences, respectively. (C) C-terminal amino acid sequence of the p67HP and BiPNHP GPI1 reporters including the HA epitope (underlined) and the EP procyclin GPI attachment peptide (black box). * indicates GPI attachment site.





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