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Fig. 6. Immunolocalization of EPMH{Delta}gpi. Bloodstream cells expressing EPMH{Delta}gpi were mock-treated (panels A-L; -) or cultured with FMK024 for 1 hour (panels M-T; +). Cells were fixed/permeabilized, stained with the indicated antibodies, and visualized by epifluorescence microscopy. (A-D,M-P) Stained with anti-EP9 and anti-BiP antibodies. (E-L,Q-T) Stained with anti-EP9 and anti-p67 antibodies. All panels: left column, merged DIC/DAPI images; right column, merged three channel fluorescent images (arrowheads indicate lysosomal localization). DAPI staining (blue) reveals nucleus (n) and kinetoplast (k) localization (indicated in A only).





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