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Fig. 1 . Identification of FLNa as a CEACAM1-L-interacting protein by yeast two-hybrid analysis. AH109 yeast cells were co-transfected with pACT2-FLNa and pGBKT7-CC1-cyto or negative control pGBKT7-Lam, and tested for ß-GAL reporter gene activity in filter-lift assays (A) and {alpha}-GAL reporter gene activity in liquid assays (B). (C) Schematic representation of FLNa and the partial FLNa cDNA clone found in the yeast two-hybrid screen (ABD, actin binding domain; 1-24, filamin repeats; H, hinge regions).





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