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Fig. 5. FLNa-CEACAM1-L interaction inhibits wound healing. (A) Monolayers of M2, A7, M2-CC1 and A7-CC1 cells were scratched with a pipette tip and images were taken immediately, 24 and 48 hours after wounding. Bar, 100 µm. (B) Distances between wound edges after 0, 24 and 48 hours were measured. Mean wound closure after 24 and 48 hours in 4 independent experiments and standard deviation are shown. (C) Time-lapse analysis of M2, A7, M2-CC1 and A7-CC1 cells after wounding. Single cells detaching from the wound edge are labeled with an asterisk. Representative details of the supplementary Movies are shown. (D) Spontaneous cell scattering of M2, A7, M2-CC1 and A7-CC1 cells. Single cells were seeded sparsely and were allowed to grow for 10 days. Bar, 10 µm.
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