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Fig. 7. CEACAM1-L expression alters F-actin assembly and tyrosine phosphorylation in M2 and A7 cells during wound healing. Indirect immunofluorescence of F-actin (phalloidin, A) and phospho-tyrosine (p-Tyr, B) in M2, A7, M2-CC1 and A7-CC1 cells. Wounded monolayers were fixed at the indicated time points. Arrowheads indicate mature focal adhesions (B, right panel). Bars, 5 µm (C) Indirect immunofluorescence of vinculin in M2, A7, M2-CC1 and A7-CC1 cells. Monolayers were fixed 1 hour after wounding. Bar, 5 µm. (D,E) Phospho-tyrosine specific immunoprecipitations. Monolayers of M2, A7, M2-CC1 and A7-CC1 cells were either left untreated or were wounded with a multichannel pipette 1 hour prior to lysis. Equal amounts of lysates were immunoprecipitated with anti-p-Tyr mAb PY99. Blots were developed for FAK (D) and paxillin (E) (n=3).





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